CHO HCP Lipase Panel

Produktnummer ABIN7670500

Kurzübersicht für CHO HCP Lipase Panel (ABIN7670500)

Reaktivität: Hamster

Nachweismethode: Fluorometric

Applikation: Western Blotting (WB)

Proben: Cell Culture Supernatant, Cell Lysate

Produktdetails

Verwendungszweck: The detection of specific lipase proteins by Western blot, pertinent to the purification of antibody biologics from CHO cell platforms.

Spezifität: 8 antibodies specific for target lipases have been validated in CHO supernatant

Produktmerkmale: The majority of biological drugs are monoclonal antibodies and the majority of these are derived from CHO platforms. A major issue in the purification of antibodies from CHO is the persistence of lipase proteins through the bioprocess to the final drug product, with implications for product stability. HCP ELISAs measure total protein population with no resolution of individual protein identity, while mass spec is not suited to dynamic monitoring. This panel of antibodies is intended to be used for broad screening bioprocess samples for the presence of individual lipases that can then be subject to further, more specific testing, such as via an individual lipase ELISA.

Bestandteile:

• anti-PLBD2 antibody (40 µL)
• anti-mLIPA antibody (20 µL)
• anti-PPT1 antibody (20 µL)
• anti-IAH1 antibody (20 µL)
• anti-PLD3 antibody (20 µL)
• anti-hLIPA antibody (20 µL)
• anti-PLA1A antibody (20 µL)
• anti-PLBL1 antibody (20 µL)
• Opal Pre-stained Protein Standard 10-245kDa (80 µL)
• Goat anti Rabbit IgG CY5 (100 µL)
• Goat anti Mouse IgG CY5 (50 µL)

Anwendungsinformationen

Protokoll:

• Resolve CHO supernatant or lysate by SDS-PAGE and transfer to membrane for Western blot probe.
• Block membrane in Blocking Buffer for Fluorescent Western Blotting (e.g. ABIN925618) for 1 hour at room temperature with rocking.
• Probe by specific anti-lipase antibody using the entirety of the primary antibody aliquot provided into 10 mL of blocking buffer overnight at 4 °C with rocking.
• Wash membranes three times in PBS with 0.05 % Tween20
• Incubate secondary antibody 1:2000 (5 µL into 10 mL of blocking buffer) with anti-host species Cy5-conjugated secondary antibody, incubated for 1 hour with rocking at room temperature.
• Wash membranes three times in PBS with 0.05 % Tween20
• Image blot in fluorescent channel appropriate for Cy5 tag on secondary antibody and Cy2 channel to visualize Opal Pre-stained Protein Standard.

Aufbereitung der Reagenzien:

Prepare specific anti-lipase antibody using the entirety of the primary antibody aliquot provided into 10 mL of blocking buffer.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C